Dilution Calculator
C1V1 = C2V2 for single dilutions, plus a serial dilution planner that prints every transfer volume.
Add 9 mL diluent to reach 10 mL total (10-fold dilution)
= 1,000 µL stock + 9,000 µL diluent
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- Deterministic math, shown in the open
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Method notes
Assumes volumes are additive and the diluent is compatible with the stock solvent. Check final solvent percentage (for example DMSO) before adding to cells.
How dilution math works
C1V1 = C2V2
The amount of solute does not change when you dilute — only the volume does. So stock concentration × stock volume equals final concentration × final volume.
Solving for stock volume
V1 = (C2 × V2) / C1
Then add diluent up to V2 — do not add V2 on top of V1.
Serial dilutions
A 10-fold series moves 1 part into 9 parts diluent at each step. Errors compound geometrically, so mix thoroughly and change tips between tubes.
When not to use this
Percent w/v and percent v/v solutions follow the same algebra, but mass-based dilutions of viscous or volatile solvents need density correction.
What Quandra v8 adds
These calculators give you one number at a time. Quandra v8 writes the statistical analysis plan, generates R/SAS code, cross-checks assumptions against a locked reference corpus, and keeps an audit trail for regulated work.